Maize

New Research Areas For Maize Breeding

Introduction Role of Biotechnology in Maize Improvement Wild Varieties Tissue Culture

Introduction

Important areas where genetics and cytogenetics can aid in the improvement of maize are as follows

  • Mobile elements in maize populations and their implications in breeding
  • Evidence is accumulating for the presence of active mobile elements in maize breeding populations that are selected for quantitative traits, such as yield, oil or protein content.
  • Elements such as Ubiquitous (Uq) were found to be pervasive in several elite US maize populations.
  • The presence of active transposable elements can drive a limitless source of variability in the populations that can be effectively utilized by a breeder.
  • It is, therefore, imperative to examine native maize germplasm for the presence of active transposable elements and analyse their role in breeding programmes.

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Role Of Biotechnology In Maize Improvement

  • With the advent of modern molecular techniques, spectacular advances are being made in chromosomal location of important qualitative and quantitative traits in maize, construction of refined molecular linkage maps using detailed DNA alalysis, understanding species relationships through the analysis of mitochondrial and chloroplast polymorphism, and finally in genetic transformations.
  • Nevertheless, success in terms of varietal development with major emphasis on yield improvement is yet to come. For example, Restriction Fragment Length Polymorphism (RFLP) technology has many potential applications in maize breeding.
  • Maize has a high degree of polymorphism, that is probably unmatched by any other higher plant examined thus far.
  • RFLP linkage map now available in maize consists of a few hundred loci that cover all the ten chromosomes and has been correlated with conventional linkage and physical maps.
  • Some workers have demonstrated that RFLP technology in Loci (QTL) mapping , but the practical gains from such findings at the field level are yet to be witnessed.
  • However, RFLP markers are also efficiently utilized in strain identification and in measuring genetic diversity .
  • An important deterrent to take up RFLP approach to solve breeding problems in developing countries like India is the high cost involved. It is, therefore, imperative that RFLP technology be used judiciously in providing meaningful solutions to problems that are of paramount importance.

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Role Of Wild Relatives In Maize Improvement

It is essential to consider the role of related species

  • Zea diploperennis
  • Z. mexicana
  • Z. Perennis
  • Z. Luxurians
  • and genera, like Tripsacum, Coix, Chinoachne, Polytoca, Scleroachne and Trilobachne in further improvement of maize as these species not only harbour priceless genes that confer resistance to important abiotic and biotic stress, but also possess potentially useful agronomic traits.

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Application Of Tissue Culture In Maize Improvement

  • Rapid advances have been made in the field of cell and tissue culture techniques in maize
  • ever since the first source in regeneration of plants from maize tissue in 1975.
  • With several public institution and private industries showing an increased interest in the in vitro techniques, the scope of application to maize genetics and breeding is bound to expand.
  • Most of the cultivated techniques, however, were standardized in countries like USA on standard inbred lines such as A 188, B 73, Mo 17, and Black Mexican Sweet Corn.
  • India does not yet have a fact good information on the genetics of regeneration. Unfortunately, these inbred lines having problems of adaptation to the Indian conditions. Also, inbred lines, which perform under field conditions, may not respond well to culture conditions and vice versa.
  • Very limited efforts are so far made to systematically screen the important Indian maize populations and inbred lines for their callusing and regeneration abilities.
  • Maize improvement through genetic engineering is amenable only when suitable tissue culture technique is developed, and the subsequent growth and appropriate expression of the transgenics in mature can be made routine.

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